Five-mL tubes inside the magnetic stand for five min, then get rid of the buffer with a pipette and clean the beads 2× with five hundred μL of clean 80% ethanol. Right after the second clean, remove all ethanol and make sure that no ethanol is left during the samples. https://superparamagneticbeads18406.onzeblog.com/29642742/the-best-side-of-total-rna-purification-kit