In order to quantify P accumulation and P efflux in the ectomycorrhizal basidiomycete fungus Hebeloma cylindrosporum. we supplied 32P to mycelia previously grown in vitro in liquid medium. The culture had four main steps that are 1) growing the mycelium on complete medium with P. 2) transfer the mycelia into new culture solution with or without P. https://www.spidertattooz.com/Metabo-HPT-NT1865DMASTM-18V-MV-15-Gauge-Brushless-Cordless-Angled-Finish-Nailer/